The AlphaLISA™ SureFire® Ultra™ Human Phospho-STAT6 (Tyr641)/Total STAT6 assay kit is used to measure both the phosphorylation of STAT6 and total levels of endogenous STAT6 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT6 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is a member of the STAT family of proteins. In response to cytokines and growth factors binding to their receptors, STAT6 is phosphorylated by JAK kinases. Once phosphorylated, STAT6 proteins form homo or heterodimers and translocate into the nucleus, where they mediate cytokine induced gene expression. It has been demonstrated that TBK1-induced STAT6 phosphorylation is connected to the STING pathway, contributing to innate immunity's reaction to viral infection. Overexpression of STAT6 plays a direct role in tumorigenesis in leukemia, lymphoma, and prostate and colon cancers.
The Multiplex SureFire Ultra Human Phospho-STAT6 (Tyr641)/Total STAT6 Detection Kit kit is used to measure both phosphorylated and total levels of endogenous STAT6 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated DAP12 analysis, and the 545 nm (Tb) signal corresponds to the total STAT6 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Application |
Cell Signaling
|
---|---|
Assay Target |
STAT6
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Predicted Species Reactivity |
Human
|
Product Group |
Kit
|
Sample Volume |
30 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Inflammation
Oncology
|
Unit Size |
100 Assay Points
|
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
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