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Multiplex SureFire Ultra Human Phospho-STAT6 (Tyr641)/Total STAT6 Detection Kit, 100 Assay Points

The AlphaLISA™ SureFire®  Ultra™ Human Phospho-STAT6 (Tyr641)/Total STAT6 assay kit is used to measure both the phosphorylation of STAT6 and total levels of endogenous STAT6 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT6 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: MPSU-PTST6-K-HV
Unit Size: 100 Assay Points
List price: USD 1,250.00
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USD 1,250.00
USD 1,250.00 /each
Part number: MPSU-PTST6-K500
Unit Size: 500 Assay Points
List price: USD 4,223.00
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USD 0.00
USD 4,223.00 /each
Part number: MPSU-PTST6-K10K
Unit Size: 10,000 Assay Points
List price: USD 25,500.00
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USD 0.00
USD 25,500.00 /each
Part number: MPSU-PTST6-K50K
Unit Size: 50,000 Assay Points
List price: USD 80,040.00
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USD 0.00
USD 80,040.00 /each

Overview

Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is a member of the STAT family of proteins. In response to cytokines and growth factors binding to their receptors, STAT6 is phosphorylated by JAK kinases. Once phosphorylated, STAT6 proteins form homo or heterodimers and translocate into the nucleus, where they mediate cytokine induced gene expression. It has been demonstrated that TBK1-induced STAT6 phosphorylation is connected to the STING pathway, contributing to innate immunity's reaction to viral infection. Overexpression of STAT6 plays a direct role in tumorigenesis in leukemia, lymphoma, and prostate and colon cancers.

The Multiplex SureFire Ultra Human Phospho-STAT6 (Tyr641)/Total STAT6 Detection Kit kit is used to measure both phosphorylated and total levels of endogenous STAT6 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated DAP12 analysis, and the 545 nm (Tb) signal corresponds to the total STAT6 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Cell Signaling
Assay Target
STAT6
Assay Target Class
Phosphoproteins
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Method
Alpha
Molecular Modification
Phosphorylation
Predicted Species Reactivity
Human
Product Group
Kit
Sample Volume
30 µL
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Oncology
Unit Size
100 Assay Points

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Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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