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Multiplex Surefire Ultra Human and Mouse Phospho-RET (panTyr)/Total RET Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-RET (panTyr)/Total RET assay kit is used to measure both the phosphorylation of RET and total levels of endogenous RET in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure RET phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: MPSU-PTRET-K500
Unit Size: 500 Assay Points
List price: USD 4,471.00
Your price:
USD 4,471.00
USD 4,471.00 /each
Part number: MPSU-PTRET-K10K
Unit Size: 10,000 Assay Points
List price: USD 27,070.00
Your price:
USD 0.00
USD 27,070.00 /each
Part number: MPSU-PTRET-K50K
Unit Size: 50,000 Assay Points
List price: USD 84,870.00
Your price:
USD 0.00
USD 84,870.00 /each
Part number: MPSU-PTRET-K-HV
Unit Size: 100 Assay Points
List price: USD 1,322.00
Your price:
USD 0.00
USD 1,322.00 /each

Overview

Rearranged during transfection (RET or c-RET) is a receptor tyrosine kinase primarily expressed as two isoforms of 1072 & 1114 amino acids. RET activation by glial cell line-derived neurotrophic family ligands (GFL) leads to its dimerization and autophosphorylation on intracellular tyrosine residues. RET is required for the development of the nervous system and several other tissues. RET alterations have been detected in numerous human cancers. The development of highly selective and potent RET kinase inhibitors is therefore of great interest to treat RET-altered cancers.

The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-RET (panTyr)/Total RET Detection Kit is used to measure both phosphorylated and total levels of endogenous RET in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated RET analysis, and the 545 nm (Tb) signal corresponds to the total RET analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Protein Detection
Assay Points
500
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Mouse
Therapeutic Area
Oncology
Unit Size
500 Assay Points

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