Skip to main content

Multiplex Surefire Ultra Phospho (Thr821/826)/Total Rb Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex phospho-Rb (Thr821/826) + Total Rb assay kit is used to measure both the phosphorylation of Rb and total levels of endogenous Rb in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Rb phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: MPSU-PTRB-N500
Unit Size: 500 Assay Points
List price: USD 4,015.00
Your price:
USD 0.00
USD 4,015.00 /each
Part number: MPSU-PTRB-N10K
Unit Size: 10,000 Assay Points
List price: USD 24,340.00
Your price:
USD 0.00
USD 24,340.00 /each
Part number: MPSU-PTRB-N50K
Unit Size: 50,000 Assay Points
List price: USD 76,160.00
Your price:
USD 0.00
USD 76,160.00 /each
Part number: MPSU-PTRB-N-HV
Unit Size: 100 Assay Points
List price: USD 1,188.00
Your price:
USD 1,188.00
USD 1,188.00 /each


The Alpha SureFire®Ultra Multiplex p-Rb (Thr821/826) (Eu) + Total Rb ssay kit is used to measure both phosphorylated and total levels of endogenous Rb in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Rb analysis, and the 545 nm (Tb) signal corresponds to the total Rb analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.


Protein Detection
Assay Points
Assay Target Class
Assay Technology
Automation Compatible
AlphaLISA SureFire Ultra Multiplex
Detection Method
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Unit Size
100 Assay Points

Image gallery

Video gallery


1-4 of 4 Resources
Guide Icon
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Guide Icon
AlphaPlex quick start guide

This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...

Brochure Icon
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Flyer Icon
Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon