Skip to main content

Multiplex Surefire Ultra Human Phospho-ErbB3 (Tyr1289)/Total ErbB3 Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex Human Phospho-ErbB3 (Tyr1289)/Total ErbB3 assay kit is used to measure both the phosphorylation of ErbB3 and total levels of endogenous ErbB3 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ErbB3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: MPSU-PTERB3-K500
Unit Size: 500 Assay Points
List price: USD 4,223.00
Your price:
USD 4,223.00
USD 4,223.00 /each
Part number: MPSU-PTERB3-K10K
Unit Size: 10,000 Assay Points
List price: USD 25,500.00
Your price:
USD 0.00
USD 25,500.00 /each
Part number: MPSU-PTERB3-K50K
Unit Size: 50,000 Assay Points
List price: USD 80,040.00
Your price:
USD 0.00
USD 80,040.00 /each
Part number: MPSU-PTERB3-K-HV
Unit Size: 100 Assay Points
List price: USD 1,250.00
Your price:
USD 0.00
USD 1,250.00 /each


Receptor tyrosine-protein kinase (ErbB3), also known as human epidermal growth factor receptor 3 (HER3), is a membrane bound protein that is encoded by the ERBB3 gene. ErbB3 possesses a neuregulin binding domain but lacks an active kinase domain, and as a result, it can bind this ligand but cannot phosphorylate proteins to send the signal to the cell. Together with other kinase-active members of the EGF receptor family, ErbB3 forms heterodimers. Pathways that cause cell proliferation or differentiation are activated by heterodimerization. In human breast cancer and other tumors such as prostate and bladder, ErbB3 overexpression is a sign of poor patient prognosis.

The AlphaLISA SureFire Ultra Multiplex Human Phospho-ErbB3 (Tyr1289)/Total ErbB3 Detection Kit is used to measure phosphorylated ErbB3 (Tyr1289) and total levels of ErbB3 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ErbB3, and the 545 nm (Tb) signal corresponds to the total ErbB3 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.


Protein Detection
Assay Technology
Automation Compatible
AlphaLISA SureFire Ultra Multiplex
Detection Method
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Therapeutic Area
Unit Size
500 Assay Points

Image gallery

Video gallery


1-5 of 5 Resources
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

AlphaPlex quick start guide

This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...

Brochure Icon Image
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon