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AlphaLISA SureFire Ultra Multiplex Human Phospho-DAP12 (Tyr91 & total) Detection Kit, Control Lysate

Positive control lysate for AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91)/Total DAP12 Detection Kit

Feature Specification
Application Cell Signaling
Protocol Time 2h at RT

Positive control lysate for AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91)/Total DAP12 Detection Kit

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Unit Size: 1 vial
Part #:
MPSU-PTDAP12-K-L
List price
USD 159.00
Your price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

The control lysate is prepared from THP 1 cells cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 4 x10⁶ cells/mL and then treated with 0.5 mM Pervanadate for 30 minutes. Cells were then lysed with the addition of 5X Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Protocol Time
2h at RT
Shipping Conditions
Shipped in Blue Ice
Target
DAP12
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Neuroscience
Unit Size
1 vial

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