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Multiplex Surefire Ultra Human Phospho-CDK1 (Thr14)/Total CDK1 Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-CDK1 (Thr14)/Total CDK1 assay kit is used to measure both the phosphorylation of CDK1 (Thr14) and total levels of endogenous CDK1 in cellular lysates.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: MPSU-PTCDK1-K500
Unit Size: 500 Assay Points
List price: USD 4,223.00
Your price:
USD 4,223.00
USD 4,223.00 /each
Part number: MPSU-PTCDK1-K10K
Unit Size: 10,000 Assay Points
List price: USD 25,500.00
Your price:
USD 0.00
USD 25,500.00 /each
Part number: MPSU-PTCDK1-K50K
Unit Size: 50,000 Assay Points
List price: USD 80,040.00
Your price:
USD 0.00
USD 80,040.00 /each
Part number: MPSU-PTCDK1-K-HV
Unit Size: 100 Assay Points
List price: USD 1,250.00
Your price:
USD 0.00
USD 1,250.00 /each

Overview

Cyclin-dependent kinase 1 (CDK1), encoded by the CDC2 gene, is a serine/threonine kinase ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. Dysregulated activation of CDK1 leads to uncontrolled cell division and is a hallmark of cancer. Inhibition of CDK1 is an active research area, especially with new approaches involving PROTACs.

The AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Thr14)/Total CDK1 Detection Kit is used to measure phosphorylated CDK1 (Thr14) and total levels of CDK1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated CDK1, and the 545 nm (Tb) signal corresponds to the total CDK1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Protein Detection
Assay Points
500
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Oncology
Unit Size
500 Assay Points

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