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AlphaScreen C-MYC Detection Kit, 50,000 Assay Points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6760611C
Unit Size: 500 Assay Points
Part Number: 6760611M
Unit Size: 10,000 Assay Points
Part Number: 6760611R
Unit Size: 50,000 Assay Points
  • This bead kit can be used for many applications, including:

    • Protein-protein interaction assays
    • Protein-peptide interaction assays
    • C-myc tagged protein detection and quantitation
    • Protein expression
    • Binding studies

    In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is c-myc-tagged, and associates with the AlphaScreen anti-c-myc Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated c-myc probe provided in the kit.

  • Antibody Conjugates
    Streptavidin/Anti-c-Myc
    Application
    Protein-protein interactions
    Assay Points
    50000
    Automation Compatible
    Yes
    Brand
    AlphaScreen
    Species
    Human
    Unit Size
    50,000 Assay Points

Resources

1-2 of 2 Resources
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...