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AlphaLISA Mouse IgM Isotyping Detection Kit, 5,000 Assay Points

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgM antibodies in different sample matrices, including cell culture media. The IgM subtype is the first antibody found during an immune response and is responsible for agglutination and cytolytic reactions.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL527HV
Unit Size:
Part Number: AL527C
Unit Size:
Part Number: AL527F
Unit Size:
  • Formats:

    • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. Serum IgM exists as a pentamer in mammals, predominates in primary immune responses to most antigens, is the most efficient complement fixing immunoglobulin and comprises approximately 10% of normal human serum Ig content. Each of the five monomers is composed of two light chains (either kappa or lambda) and two heavy chains. IgM can cause cell agglutination as a result of recognition of epitopes on invading microorganisms. This antibody-antigen immune complex is then destroyed by complement fixation or receptor mediated endocytosis by macrophages. Typically mouse serum and plasma samples contain about 0.5 to 6.5 mg/mL of IgM. The present kit permits detection of mouse IgM (i.e. analyte) in different sample matrices, including different cell culture media and monkey serum.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Biologics/Bioprocess
    Protein Analysis & Detection
    Assay Target Class
    Antibody
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates