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AlphaLISA Mouse IgG1 Isotyping Detection Kit, 100 Assay Points

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL522HV
Unit Size: 100 Assay Points
List price: USD 1,095.00
Your price:
USD 1,095.00
USD 1,095.00 /each
Part number: AL522C
Unit Size: 500 assay points
List price: USD 2,299.00
Your price:
USD 0.00
USD 2,299.00 /each
Part number: AL522F
Unit Size: 5,000 Assay Points
List price: USD 15,220.00
Your price:
USD 0.00
USD 15,220.00 /each

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically mouse serum and plasma samples contain about 7 to 10 mg/ml of IgG.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Biologics/Bioprocess
Protein Analysis & Detection
Assay Target Class
Antibody
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Quantity in a Package Amount
100.0 Units
Shipping Conditions
Shipped in Blue Ice
Unit Size
100 Assay Points

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