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LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points

LANCE Ultra phospho-SMAD2 (Ser465/467) kits are designed for the detection of SMAD2 (phosphorylated at S465/S467) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: TRF4012C
Unit Size: 500 Assay Points
List price: USD 2,043.00
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USD 2,043.00
USD 2,043.00 /each
Part number: TRF4012M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
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USD 0.00
USD 9,232.00 /each

Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4012S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

SMAD proteins are transcriptional activators activated by bone morphogenic proteins (BMPs). These ligands induce dimerization of SMAD proteins, whereby they translocate to the nucleus and bind target genes. SMAD1, 2, and 3 constitute receptor-regulated SMADs.

Specifications

Application
Cell Signaling
Assay Pathway
TGFß/SMAD
Assay Target
SMAD2
Assay Target Class
Phosphoproteins
Assay Technology
TR-FRET
Automation Compatible
Yes
Brand
LANCE Ultra
Cellular Process
TGFß/SMAD
Detection Method
LANCE
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
15 µL
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Metabolic
Oncology
Respiratory
Unit Size
500 Assay Points

Resources

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

Application Note Icon
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...

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