Skip to main content
Menu
US

LANCE Ultra Mouse IL-6 Detection Kit, 500 Assay Points

The LANCE® Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF1504C
Unit Size: 500 Assay Points
List price: USD 1,134.00
Your price:
USD 1,134.00
USD 1,134.00 /each
Part number: TRF1504M
Unit Size: 10,000 Assay Points
List price: USD 15,010.00
Your price:
USD 0.00
USD 15,010.00 /each

Overview

The LANCE Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

In the mouse, Interleukin 6 (IL6) is a 187 amino acid cytokine. In humans, IL6 is produced by a variety of cell types, including T-cells, mast cells, and monocytes. IL6 signals through binding to the gp130/ IL6R receptor complex. IL6 acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or antiinflammatory responses, depending on the target cell analyzed and the in vivo environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver and acts as a survival factor on neuronal cells. Elevated serum IL6 levels have been observed during bacterial and viral infection, trauma, autoimmune disease, inflammation, and cancer.

Specifications

Application
Protein Analysis & Detection
Assay Points
500
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
LANCE Ultra
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

Resources

1-1 of 1 Resources
Guide Icon
Guide
Download your guide about neurodegenerative diseases

Emerging pathways to neuroinflammation and neurodegeneration

Neurodegenerative diseases, such as amyotrophic lateral sclerosis...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon