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LANCE Ultra Human Frataxin Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF1322C
Unit Size: 500 Assay Points
List price: USD 1,136.00
Your price:
USD 0.00
USD 1,136.00 /each
Part number: TRF1322M
Unit Size: 10,000 Assay Points
List price: USD 15,010.00
Your price:
USD 15,010.00
USD 15,010.00 /each

Overview

The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Frataxin is expressed as a 210 AA, 23 kDA protein from the FXN gene located on chromosome 9. Upon expression, the FXN protein is directed to the mitochondrion by its 41 AA N-terminal mitochondrial targeting sequence. In the mitochondrion, the protein is cleaved by the mitochondrial processing peptidase (MPP) to its intermediate form of 42 – 210 AA. Later on, MPP cleaves the protein to its mature form of 81-210 AA. Although the function of the FXN protein is not clearly defined, it is thought to be vitally important for Iron-Sulfur cluster biogenesis, heme biosynthesis, and chelation and transportation of iron specifically involved with the mitochondria. The Frataxin protein is the primary culprit for a debilitating neurodegenerative disease called Friedreich’s Ataxia. Due to similarities in the diseases, there is some evidence to suggest that FXN may be involved in other neurodegenerative diseases such as Parkinson’s, Multiple Sclerosis, and Amyotrophic Lateral Sclerosis. This kit has been designed for the detection and quantification of FXN from cell and tissue lysates.

Specifications

Application
Protein Analysis & Detection
Assay Points
10000
Assay Target Class
Protein
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Format
Microplates
One Unit Contains
10000.0 assay points
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Neuroscience
Unit Size
10,000 Assay Points

Resources

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Guide
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Emerging pathways to neuroinflammation and neurodegeneration

Neurodegenerative diseases, such as amyotrophic lateral sclerosis...

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SDS, COAs, Manuals and more

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