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LANCE Ultra CCL4 Detection Kit, 500 Assay Points

The LANCE® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF1258C
Unit Size: 500 Assay Points
Part Number: TRF1258M
Unit Size: 10,000 Assay Points
  • The LANCE®Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human CCL4/MIP-1ß in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    • No-wash steps, no separation steps
    • TR-FRET technology
    • Sensitive detection
    • High reproducibility
    • Faster time-to-results
    • Easy automation
    • 96-well, 384-well, and 1536-well formats

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

    C-C Motif Chemokine 4 (CCL4), also known as Macrophage Inflammatory Protein-1beta (MIP-1ß), is a 69 amino acid chemokine signaling through the CCR5 receptor. It is a chemoattractant for natural killer cells, monocytes, and a variety of other immune cells. CCL4 is also an important regulator of macrophage migration. CCL4 is rapidly synthesized by activated T cells, B cells, and monocytes. It is also produced by neutrophils after adhesion to the laminin contained in the basement membrane of blood vessels. It is one of the major HIV-suppressive factors produced by CD8+ T-cells and has been reported to show a direct antiviral activity against HSV-1. Recombinant CCL4 also induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus.

  • Application
    Protein Analysis & Detection
    Assay Points
    500
    Assay Target
    CCL4
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity in a Package Amount
    1.0 Units
    Therapeutic Area
    Inflammation
    Unit Size
    500 Assay Points