Skip to main content

LANCE Ultra cAMP Detection Kit, 10,000 Assay Points

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF0262
Unit Size: 1,000 Assay Points
Part Number: TRF0263
Unit Size: 10,000 Assay Points
Part Number: TRF0264
Unit Size: 50,000 assay points
  • LANCE Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors. Features: Unmatched assay sensitivity and signal stability-even with overnight incubation. Optimized screening of difficult targets and Gαi antagonists. Easier detection of cAMP response of endogenous receptors-use fewer cells per well. Robust sensitivity when miniaturized to 1536-well format uHTS. Trusted results: stable pharmacology over time with consistent rank order potencies. Reproducible results with highest values LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

    assay is based on competition between europium (Eu) chelate-labeled cAMP tracer and cellular cAMP binding sites on cAMP-specific monoclonal antibodies labeled with ULight dye. When antibodies are bound to Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites Eu chelate molecule of tracer. energy emitted by excited Eu chelate is transferred by FRET to ULight molecules on antibodies, which in turn emit light at 665 nm.

  • Application
    cAMP
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Format
    Microplates
    Second Messenger Release
    cAMP
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Metabolic
    Unit Size
    10,000 Assay Points