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LANCE Ultra cAMP Detection Kit, 10,000 Assay Points

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Second Messenger Detection
Sample Volume 10 µL

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 1,000 Assay Points
Part #:
TRF0262
List Price
USD 907.39
Unit Size: 10,000 Assay Points
Part #:
TRF0263
List Price
USD 2,809.00
Unit Size: 50,000 assay points
Part #:
TRF0264
List Price
USD 11,750.00

Overview

LANCE Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors. Features: Unmatched assay sensitivity and signal stability-even with overnight incubation. Optimized screening of difficult targets and Gαi antagonists. Easier detection of cAMP response of endogenous receptors-use fewer cells per well. Robust sensitivity when miniaturized to 1536-well format uHTS. Trusted results: stable pharmacology over time with consistent rank order potencies. Reproducible results with highest values LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

assay is based on competition between europium (Eu) chelate-labeled cAMP tracer and cellular cAMP binding sites on cAMP-specific monoclonal antibodies labeled with ULight dye. When antibodies are bound to Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites Eu chelate molecule of tracer. energy emitted by excited Eu chelate is transferred by FRET to ULight molecules on antibodies, which in turn emit light at 665 nm.

Specifications

Application
Second Messenger Detection
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Modality
LANCE
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
cAMP
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Metabolic
Unit Size
10,000 Assay Points

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

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Guide
LANCE Ultra cAMP Assay development guidelines

The LANCE™ Ultra cAMP Assay Development Guidelines provide comprehensive instructions for the quantitative determination of cyclic...

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