The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 101.3- 300,000 pg/mL |
Limit of Detection | 101.3 pg/mL |
Limit of Quantification | 724.6 pg/mL |
Sample Volume | 15 µL |
The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein (ANGPTL4) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Angiopoietin-like 4 (ANGPTL4), also known as HFARP, PGAR, or FIAF, is a 55 kDa glycoprotein that is structurally related to the angiopoietin family. It is secreted by the liver and fat tissue, and contains an N-terminal coiled-coil domain, and taC-terminal fibrinogen-like domain. Angiopoietin-like 4 plays a role in lipoprotein metabolism and angiogenesis. It is also involved in numerous cancers and is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and invasiveness.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Modality |
LANCE
|
Dynamic Range |
101.3- 300,000 pg/mL
|
Limit of Detection |
101.3 pg/mL
|
Limit of Quantification |
724.6 pg/mL
|
Product Group |
Kit
|
Sample Volume |
15 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
ANGPTL4
|
Target Class |
Biomarkers
|
Target Species |
Human
|
Technology |
TR-FRET
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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