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LANCE Streptavidin Fluorophore tag, 50µg

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 50 µg
Part #:
AD0062
List Price
USD 1,485.00
Unit Size: 1 mg
Part #:
AD0063
List Price
USD 12,910.00

Overview

Isolated from fermentation filtrates of Streptomyces avidinii, 1 mg of streptavidin binds approximately 10 µg of biotin. The preparations are supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
LANCE
Conjugates
Streptavidin
Detection Modality
LANCE
Fluorophore
Eu-W1024
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Blue Ice
Target Class
Binding Assay
Technology
TR-FRET
Unit Size
50 µg

Resources

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Application Note Icon
Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

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