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LANCE Eu-W1024-ITC Chelate and Eu Standard, 0.2 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Labeling

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variant
Unit Size: 0.2 mg
Part #:
AD0096
List Price
USD 3,716.00

Overview

LANCE® Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).

  • Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as ones a N-terminus of protein or lysine residues. 
  • LANCE® W1024 Series chelates are preferred in most HTS applications due to their lower fluorescent background in TR-FRET assays. 
  • For most LANCE™ applications the W1024 series of Eu chelates with active group can be used. Usable pH range 5-11. Under neutral conditions W1024 remains stable in EDTA concentrations up to 20 mmol/L. Allophycocyanin (APC)- or ULight-labeled compounds can be used as acceptors for LANCE® TR-FRET assays.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Labeling
Automation Compatible
Yes
Brand
LANCE
Detection Modality
LANCE
Fluorophore
Eu-W1024-ITC
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Blue Ice
Technology
TR-FRET
Unit Size
0.2 mg

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LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

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