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LANCE Eu-W1024 Anti-Human IgG Antibody, 10µg

Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AD0212
Unit Size: 10 µg
List price: USD 300.01
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USD 300.01
USD 300.01 /each
Part number: AD0074
Unit Size: 50 µg
List price: USD 1,486.00
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USD 0.00
USD 1,486.00 /each
Part number: AD0075
Unit Size: 1 mg
List price: USD 12,910.00
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USD 0.00
USD 12,910.00 /each

Overview

Eu-W1024 labeled anti-human IgG antibodies for LANCE® assays where analyte concentration is < 1-10 nmol/L and assay pH > 7. This affinity purified mouse monoclonal IgG2b antibody is specific for the Fc-part of human IgG. The antibodies recognize all IgG subclasses and cross-react with rabbit immunoglobulins. It is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates
Anti-human IgG
Application
Protein Analysis & Detection
Automation Compatible
Yes
Brand
LANCE
Detection Method
TR-FRET
Fluorophore
Eu-W1024
Format
Microplates
One Unit Contains
10.0 µg
Shipping Conditions
Shipped in Blue Ice
Unit Size
10 µg

Citations

Resources

1-1 of 1 Resources
Application Note Icon
Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...

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