Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
This is a replacement for AD0111.
Feature | Specification |
---|---|
Application | Protein-Protein Interaction |
Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
This is a replacement for AD0111.
Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
LANCE (Lanthanide chelate excite) and LANCE® Ultra™ are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
Application |
Protein-Protein Interaction
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Conjugates |
Anti-6X His
|
Detection Modality |
LANCE
|
Fluorophore |
Eu-W1024
|
Product Group |
Antibody
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Binding Assay
|
Technology |
TR-FRET
|
Unit Size |
10 µg
|
Are you looking for resources, click on the resource type to explore further.
Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...
LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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