Harmony High-Content Imaging and Analysis Software

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

• Phenotypic Screening
  • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
  • Easily train the software to recognize relevant phenotypes
  • Quantify even subtle phenotypic differences
• Live Cell Imaging
  • Follow phenotypic changes over time
  • Accurately quantify label-free live cell images
  • Track individual cells over time or analyze wound-healing assays
  • Analyze cell signaling dynamics using e.g. FRET based assays
  • Perform fast response assays such as calcium flux or cardiomyocyte beat rate with imaging frame rate of up to 100fps.
  • Set-up "dispense and read" assays on the Opera Phenix Plus system with liquid handling module
  • Enhance your live-cell assay capabilities through improved time-series flexibility
• 3D Cell Models
  • Speed up 3D image acquisition by targeted imaging of microtissues
  • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
  • Measure morphologies and volumes in 3D, count nuclei within spheroids
  • Enhance Maximum Intensity Projection Analysis using PlaneMap
  • One software for image acquisition, 3D visualization and analysis
• Rare Cell Phenotypes
  • Quickly capture your rare cell phenotypes with PreciScan™
  • Pre-screen at low resolution and acquire objects of interest at high resolution
  • Shorten your image acquisition times
  • Reduce your data volume
• Routine Assays
  • Utilize more than 30 ready-made-solutions for standard assays
  • Ensure repeatability by running established protocols
• Optional modules
  • Phenologic.AI offers an efficient and reliable method for identifying cells and cellular nuclei within fluorescent and brightfield images by harnessing the power of pre-trained deep neural networks (DNNs).
  • Connect Harmony software to your favorite image analysis tools. Access greater flexibility with the Harmony Application Connector. Integrate third-party applications, including Cellpose, to access advanced AI-driven analysis and user trained AI-models maintaining a streamlined workflow within the Harmony environment.^1,2
    
    

One high-content analysis software for all users

• New Users
  • Get quantification for your cell images
  • Easy to learn – even for users with little microscopy or programming knowledge
  • Get started with 30 ready-made solutions
  • Logical workflow for easy control of all aspects of image acquisition and analysis
  • Simple building blocks to assemble image analysis sequences (step-by-step)
• Expert Users
  • Quantify difficult phenotypes with advanced texture and morphology readouts
  • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
  • Create “stitched” global images from multiple fields of view
  • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
• For Lab Managers
  • Keep up with increasing research needs – analyze larger sample sizes
  • Quickly train new users to set up the instrument and analyze their images
  • Find images, metadata and results quickly via the integrated sortable database
  • View and analyze data from any computer with installation of Harmony software
  • Get application support by image analysis experts

¹Third-party applications are provided and licensed separately by their respective owners. Customers are responsible for obtaining, licensing, and deploying any third-party software used with the Harmony Application Connector and for complying with all applicable license terms.

²Cellpose is a third-party open source tool and is not affiliated with or endorsed by Revvity. Cellpose is developed by MouseLand / Howard Hughes Medical Institute.