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LANCE Ultra Anti-Phospho-p70 S6K (Thr389) Antibody, 15,625 Assay Points

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF0214-C
Unit Size: 1.7 µg
List price: USD 2,183.00
Your price:
USD 0.00
USD 2,183.00 /each
Part number: TRF0214-D
Unit Size: 10 µg
List price: USD 2,911.00
Your price:
USD 0.00
USD 2,911.00 /each
Part number: TRF0214-M
Unit Size: 100 µg
List price: USD 9,966.00
Your price:
USD 9,966.00
USD 9,966.00 /each


LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody

  • 10 μg = 1,562 assay points
  • 100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.


Antibody Conjugates
Automation Compatible
Detection Method
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Molecular Modification
One Unit Contains
100.0 µg
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Unit Size
100 µg
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