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LANCE Ultra Anti-Phospho-Topoisomerase 2a (Thr1342) Antibody, 1,562 Assay Points

Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: TRF0218-D
Unit Size: 10 µg
List price: USD 2,911.00
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USD 2,911.00
USD 2,911.00 /each
Part number: TRF0218-M
Unit Size: 100 µg
List price: USD 9,966.00
Your price: Login to view
USD 0.00
USD 9,966.00 /each

Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody

  • 10 μg = 1,562 assay points
  • 100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Antibody Conjugates
Anti-phospho-Topo IIa
Application
Biochemical Enzymatic Assay
Assay Target Class
Kinase
Assay Technology
TR-FRET
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
LANCE
Fluorophore
Eu-W1024
Molecular Modification
Phosphorylation
Product Group
Antibody
Shipping Conditions
Shipped in Blue Ice
Unit Size
10 µg

Resources

1-2 of 2 Resources
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Guide
Finding the pathway to assay optimization is easy

Comprehensive Kinase and Antibody Selection for LANCE Ultra Assays

Our comprehensive guide helps you identify and utilize kinases...

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

SDS, COAs, Manuals and more

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Certificate of analysis
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