Skip to main content
Menu
US

LANCE Ultra Anti-Phospho-4EBP1 (Thr37/46) Antibody, 1,562 Assay Points

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF0216-D
Unit Size: 10 µg
List price: USD 2,911.00
Your price:
USD 2,911.00
USD 2,911.00 /each
Part number: TRF0216-M
Unit Size: 100 µg
List price: USD 9,966.00
Your price:
USD 0.00
USD 9,966.00 /each

Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody

  • 10 μg = 1,562 assay points
  • 100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Antibody Conjugates
Anti-phospho-4EBP1
Application
Kinase
Assay Target Class
Phospho-protein
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Fluorophore
Eu-W1024
Format
Microplates
Molecular Modification
Phosphorylation
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Metabolic
Unit Size
10 µg
SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon