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LANCE Ultra Anti-K4 Histone H3 Antibody, 10µg

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF0404-D
Unit Size: 10 µg
Part Number: TRF0404-M
Unit Size: 100 µg
  • The LANCE® Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) antibody was used for the development and optimization of a SIRT1 deacetylation assay using a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4 as substrate. A technical note describing the assay is available in our product literature.

    Formats:

    • 10 µg = 1,500 assay points (384-well assay format)
    • 100 µg = 15,000 assay points (384-well format)

    Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

    Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

  • Automation Compatible
    Yes
    Application
    Epigenetics
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Fluorophore
    Eu-W1024
    Format
    Microplates
    Molecular Modification
    Acetylation
    Methylation
    Therapeutic Area
    Oncology
    Antibody Conjugates
    Anti-H3K4
    Brand
    LANCE Ultra
    Unit Size
    10 µg