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DELFIA Eu-Labeling Kit (Eu-N1-ITC Chelate), 0.2mg

The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 1244-303
Part Number: 1244-303
  • Each kit contains 0.2 mg labeling reagent plus Sm-standard, DELFIA Enhancement Solution, Sm-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.

    N1 chelates can be used in DELFIA separation assays where the concentration of chelating agents is < 0.05 mmol/L, pH >7, and the temperature is < 38°C. They are suitable for highly sensitive end point measurements in separation based assays such those for detection of protein-protein binding and cell adhesion as well as for immunoassays. Isothiocyanate activated chelates react primarily with free lysines on the compound to be labeled.

  • Application
    Protein-protein interactions
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    DELFIA
    Detection Method
    DELFIA TRF
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Fluorophore
    Sm-N1-ITC
    Format
    Microplates
    Quantity in a Package Amount

Resources

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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development
biotherapeutics, delfia, trf, time-resolved fluorescence
Guide
DELFIA immunoassay development guide
delfia, guide, protocol, assay, immunoassay
Guide
Time-resolved fluorometry with the DELFIA method user guide
delfia, guide, fluorometry, assays, trf technology