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DELFIA Inducer, 250mL

DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 4013-0010
Part Number: 4013-0010
  • DELFIA® Inducer is a ready-for-use solution with Triton X-100, glycine, hydrochloric acid and chelators. It is intended for use in the rapid quantitative determination of Eu3+ and Sm3+ in time-resolved fluorometric assays. It is suitable for use in multilabel assays that require DELFIA® Enhancer for development of Tb3+ and Dy3+ fluorescence. The solution dissociates lanthanide from solid phase bound DELFIA® chelate labeled biomolecules in a few minutes depending from used chelate to form a homogeneous and highly fluorescent Eu/Sm-(BFPP)3(TOPO)2-3 micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be performed when using a time-resolved fluorometer.

    For use with stable chelates such as DTPA, to provide more rapid signal development. Suitable for use in all multianalyte assays.

  • Application
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    DELFIA
    Detection Method
    DELFIA TRF
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Format
    Microplates
    Unit Size
    250 mL

Resources

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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.