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DELFIA Eu-Labeling Kit (Eu-N1-ITC Chelate), 0.4mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 1244-302
Part Number: 1244-302
  • The DELFIA Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays. Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as those at the N-terminus of protein or lysine residues.

    Each kit contains 0.4 mg labeling reagent plus Eu-standard, DELFIA Enhancement Solution, Eu-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.

  • Application
    Protein-protein interactions
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    DELFIA
    Detection Method
    DELFIA TRF
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Fluorophore
    Eu-N1-ITC
    Format
    Microplates
    Quantity in a Package Amount
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    1 Kit

Resources

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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide
DELFIA immunoassay development guide

DELFIA immunoassays are a superior alternative to traditional ELISAs

Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.