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DELFIA Eu-N1 Anti-6His Antibody, 1mg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

This is a replaced for AD0108.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AD0310
Unit Size:
Part Number: AD0309
Unit Size:
  • Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.

    DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of auto fluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

  • Antibody Conjugates
    Anti-6X His
    Assay Technology
    Time-Resolved Fluorescence (TRF)
    Automation Compatible
    Yes
    Brand
    DELFIA
    Detection Method
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Fluorophore
    Eu-N1

Resources

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Application Note Icon Image
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development
biotherapeutics, delfia, trf, time-resolved fluorescence
Guide
DELFIA immunoassay development guide
delfia, guide, protocol, assay, immunoassay
Guide
Time-resolved fluorometry with the DELFIA method user guide
delfia, guide, fluorometry, assays, trf technology