Skip to main content
Menu
US

AlphaScreen Biotinylated-HIS

Biotinylated-His peptide, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA™, AlphaScreen®, or AlphaPlex™) no-wash assays. This product contains 0.5 mL of biotinylated-His at 5 µM. This probe can be used with either Nickel chelate beads or Anti-His antibody-coated beads.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW when you buy online.
Promo code: REV15NOW
Offer valid until 11/6. Terms and conditions apply.
Part number: 6760303M
Unit Size: 0.5 mL
List price: USD 792.00
Your price: Login to view
USD 792.00
USD 792.00 /each

Overview

AlphaScreen® and AlphaLISA™ are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Application
Protein-Protein Interaction
Assay Target Class
Binding Assay
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaScreen
Detection Method
Alpha
Product Group
Standard
Species
Human
Shipping Conditions
Shipped in Blue Ice
Unit Size
0.5 mL

Video gallery

Resources

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Certificate of analysis
+ Show more
Scroll Icon