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AlphaScreen Anti-Rabbit IgG Alpha Donor Beads, 25 mg

Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AS105D
Unit Size: 1 mg
List price: USD 757.38
Your price:
USD 0.00
USD 757.38 /each
Part number: AS105M
Unit Size: 5 mg
List price: USD 2,957.00
Your price:
USD 0.00
USD 2,957.00 /each
Part number: AS105R
Unit Size: 25 mg
List price: USD 13,750.00
Your price:
USD 13,750.00
USD 13,750.00 /each

Overview

Alpha Donor beads coated with anti-rabbit IgG antibody. The conjugated goat polyclonal F(ab')2 fragment targets the Fc region of rabbit IgG. These beads can be used to capture rabbit IgG antibodies, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Antibody detection assays
  • Antibody binding assays
  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Antibody Conjugates
Anti-rabbit IgG
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Bead Type or Material
Alpha Donor
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
One Unit Contains
25.0 mg
Shipping Conditions
Shipped in Blue Ice
Unit Size
25 mg

Video gallery

Resources

1-4 of 4 Resources
Application Note Icon
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions

This note describes development of an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear...

Application Note Icon
Application Note
A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions

Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive...

Guide Icon
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A

Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.

Therapeutic...

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SDS, COAs, Manuals and more

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