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AlphaLISA Anti-Human IgG3 Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to a anti-human IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL155C
Unit Size: 250 µg
Part Number: AL155M
Unit Size: 5 mg
Part Number: AL155R
Unit Size: 25 mg
  • Features:

    • No-wash steps, no separation steps
    • Ease-of-use: few addition steps, fast assay development
    • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
    • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
    • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

    Immunoglobulin G (IgG) is a major effector molecule of the humoral immune response and accounts for about 75% of the total immunoglobulins in plasma of healthy individuals. The remainder 25% comprises IgM, IgA, IgD and IgE, each of which has characteristic properties and functions. The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by inter-chain disulfide bonds. Four IgG subclasses have been identified: IgG1, IgG2, IgG3 and IgG4.

    These beads can be used in conjunction with Alpha Donor beads for use in AlphaLISA no-wash assays for isotyping or antibody binding studies. In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

    AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

  • Antibody Conjugates
    Anti-human IgG3
    Application
    Isotyping
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Bead Type or Core Bead Type
    AlphaLISA Acceptor
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Species
    Human
    Rat
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    250 µg

Resources

1-4 of 4 Resources
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.