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AlphaScreen Anti-FLAG Donor Beads, 5 mg

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AS103D
Unit Size: 1 mg
Part Number: AS103M
Unit Size: 5 mg
Part Number: AS103R
Unit Size: 25 mg
  • Alpha Donor beads conjugated to anti-FLAG antibody. The mouse monoclonal antibody M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites. These beads can be used to capture FLAG-tagged antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

    • Analyte detection assays
    • Protein-protein interaction assays
    • Protein-DNA interaction assays
    • Protein-RNA interaction assays
    • Protein-small molecule interaction assays
    • Protein detection assays
    • Enzymatic assays

    In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

  • Antibody Conjugates
    Anti-FLAG
    Application
    Protein-protein interactions
    Automation Compatible
    Yes
    Bead Type or Core Bead Type
    Alpha Donor
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    5 mg

Resources

1-6 of 6 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions

This note describes development of an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.

Fc Gamma Receptors (FcγRs) are members of the immunoglobulin superfamily and play a critical role in the function of therapeutic...

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.