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AlphaPlex 545 (Terbium) Human IL-2 Detection Kit, 100 Assay Points

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AP221TB-HV
Unit Size:
Part Number: AP221TB-C
Unit Size:
Part Number: AP221TB-F
Unit Size:
  • The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

    Formats:

    • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

    AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

  • Application
    Multiplexing
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    AlphaPlex
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Inflammation

Resources

1-3 of 3 Resources
Guide
AlphaPlex quick start guide
alphaplex, guide, overview, assays, technology
Flyer
Measure more analytes in each well.
alphaplex, multiplexing, flyer, acceptor beads, donor beads, beads
Application Note Icon Image
Application Note
Simultaneous Detection of IL-6 and IL-8 Secretion by Cell Lines using AlphaPlex Technology
alphaplex, multiplexing, il-6, il-8, cytokines