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AlphaPlex 545 (Terbium) Human IL-17 Detection Kit, 100 assay points

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AP219TB-HV
Unit Size: 100 Assay Points
List price: USD 564.36
Your price:
USD 564.36
USD 564.36 /each
Part number: AP219TB-C
Unit Size: 500 Assay Points
List price: USD 2,390.00
Your price:
USD 0.00
USD 2,390.00 /each
Part number: AP219TB-F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price:
USD 0.00
USD 15,950.00 /each

Overview

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit contains reagents to run 100 assays, using a 100 µL reaction volume (10 µL of sample). Protocol can be found on the Certificate of Analysis.

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Specifications

Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaPlex
Detection Method
Alpha
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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Guide
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Application Note
Simultaneous Detection of IL-6 and IL-8 Secretion by Cell Lines using AlphaPlex Technology

This application note describes how AlphaPlex reagents from Revvity can be used to simultaneously detect IL-6 and IL-8 secretion...

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