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AlphaLISA Human Osteoprotegerin Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 0.26 - 30,000 pg/mL
Limit of Detection 0.26 pg/mL  
Limit of Quantification 0.87 pg/mL  
Sample Volume 10 µL

The AlphaLISA™ immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL3010HV
List Price
USD 971.37
Unit Size: 500 Assay Points
Part #:
AL3010C
List Price
USD 4,207.00
Unit Size: 5,000 Assay Points
Part #:
AL3010F
List Price
USD 29,270.00

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor, is a human protein encoded by the gene TNFRSF11B (tumor necrosis factor receptor superfamily member 11B). It is a cytokine decoy receptor for nuclear factor kappa B ligand receptor, and neutralizes its function in osteoclastogenesis. Osteoprotegerin-mediated pathways maintain the structure of healthy bone by inhibiting osteoclast activation. It is released as a disulfide-linked homodimer, though the monomeric form is also present in human serum. The OPG pathway poses a potential therapy for osteopenic disorders, such as osteoporosis, arthritis and hypercalcemia. This AlphaLISA kit will detect both the homodimer and the monomeric forms of osteoprotegerin.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
0.26 - 30,000 pg/mL
Limit of Detection
0.26 pg/mL  
Limit of Quantification
0.87 pg/mL  
Molecular Modification
Total
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
Osteoprotegerin
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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