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AlphaLISA Human SHP-2 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for human SHP-2 enables the quantitative determination of human Src-homology domain-2 containing protein tyrosine phosphatase 2 (SHP-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 4.2 - 300,000 pg/mL  
Limit of Detection 4.2 pg/mL  
Limit of Quantification 15.6 pg/mL  
Sample Volume 10 µL

The AlphaLISA™ immunoassay kit for human SHP-2 enables the quantitative determination of human Src-homology domain-2 containing protein tyrosine phosphatase 2 (SHP-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL3074HV
List Price
USD 1,145.00
Unit Size: 500 Assay Points
Part #:
AL3074C
List Price
USD 2,750.00
Unit Size: 5,000 Assay Points
Part #:
AL3074F
List Price
USD 22,240.00

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

SHP-2 is Src-Homology domain-2 containing protein tyrosine Phosphatase 2, also known as PTPN11, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, protein tyrosine phosphatase, non-receptor type 11. It is a tyrosine phosphatase which dephosphorylates tyrosine residues in proteins. It is widely expressed in most tissues and acts as signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Mutations in this gene have been linked f Noonan syndrome, LEOPARD syndrome and childhood leukemia. The association with breast cancer, leukaemia, lung cancer, liver cancer, gastric cancer, laryngeal cancer, oral cancer and other cancer types makes SHP-2 useful as a tumor biomarker. This kit has been designed for the detection of human SHP-2 in cell culture media and cell lysate.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
4.2 - 300,000 pg/mL  
Limit of Detection
4.2 pg/mL  
Limit of Quantification
15.6 pg/mL  
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
SHP2
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Therapeutic Area
Metabolic
Unit Size
100 Assay Points

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