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AlphaLISA Human PON-1 Detection Kit, 500 Assay Points

The AlphaLISA™ immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 28.5 - 300,000 pg/mL  
Limit of Detection 28.5 pg/mL  
Limit of Quantification 107.2 pg/mL  
Sample Volume 5 µL

The AlphaLISA™ immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL3071HV
List Price
USD 1,145.00
Unit Size: 500 Assay Points
Part #:
AL3071C
List Price
USD 2,750.00
Unit Size: 5,000 Assay Points
Part #:
AL3071F
List Price
USD 22,240.00

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Paraoxonase/arylesterase 1 (PON-1) is a serum enzyme typically only found in mammals. Like most of the paraoxonase family its main function is to act as anti-atherosclerotic component of high-density lipoproteins (HDL). PON-1 levels vary between individuals and has shown to be affected by diet and life-style. PON-1 has shown enzymatic activity against a wide variety of drug classes and toxins including: glucoronides, thiolactones, arylesters, cyclic carbonates, organophosphorous pesticides and nerve gases.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
28.5 - 300,000 pg/mL  
Limit of Detection
28.5 pg/mL  
Limit of Quantification
107.2 pg/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
PON1
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Unit Size
500 Assay Points

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