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AlphaLISA Mouse PD1 / PDL1 Binding Kit, 5,000 Assay Points

The AlphaLISA™ mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 5 µL

The AlphaLISA™ mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
AL580C
List Price
USD 3,338.00
Unit Size: 5,000 Assay Points
Part #:
AL580F
List Price
USD 16,580.00

Overview

Basic immunoregulation studies using human cells can be challenging, whereas options for mouse cells are extensive (immunization of donor mice to generate T cells, T cell receptor transgenic mice). It can often be quicker to learn about immunology and regulation of immune responses by using a mouse system. However, working in mouse systems can often require developing separate mouse reagents if your human drug or antibody won't cross-react with mouse. The AlphaLISA mouse PD-1/PD-L1 binding kit allows determination of cross-reactivity of human anti-PD-1 or PD-L1 reagents with mouse proteins, as well as development or screening of novel mouse PD-1 and PD-L1 reagents that block the binding of this interaction. Using the AlphaLISA mouse PD-1/PD-L1 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format. Please note an AlphaLISA human PD-1/PD-L1 binding assay is also available for concurrent studies.

AlphaLISA Binding Assay Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad range of affinities
  • High avidity
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of binding interactions in a highly sensitive, quantitative, reproducible and user-friendly mode. In the AlphaLISA assay, a biotinylated mouse PD-L1 binds to the Streptavidin-coated Alpha Donor beads, while His-tagged mouse PD-1 is captured by Anti-6X-His AlphaLISA Acceptor beads. When PD-1 binding to PD-L1 occurs, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
PD-1, PD-L1
Target Class
Binding Assay
Target Species
Mouse
Technology
Alpha
Therapeutic Area
Immuno-oncology
Unit Size
5,000 Assay Points

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Addressing the challenges of bispecific antibody characterization with high-throughput platforms

Bispecific antibodies (bsAbs) have emerged as an attractive class of therapeutic agents with the potential to revolutionize the...

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