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AlphaLISA Mouse MMP-9 Detection Kit, 500 Assay Points

The AlphaLISA™ Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 19.9 - 300,000 pg/mL  
Limit of Detection 19.9 pg/mL  
Sample Volume 5 µL

The AlphaLISA™ Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
AL519C
List Price
USD 4,585.00
Unit Size: 5,000 Assay Points
Part #:
AL519F
List Price
USD 35,780.00

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Mouse Matrix Metalloproteinase-9 (MMP9) or gelatinase B is a precursor maturing into a 69 kDa, zinc- and calciumdependent, endopeptidase secreted by normal alveolar macrophages and granulocytes. MMP9 is secreted as a proenzyme (pro-MMP9) and is activated by proteolytic cleavage by serine proteases including other MMPs. In human, MMP9 is believed to act synergistically with MMP1 interstitial collagenase in the degradation of fibrillar collagens. MMP9 might contribute to the pathogenesis of joint destruction. MMP9 could also play a role in bone osteoclastic resorption and cancer metastasis. MMP9 constitutes a useful biomarker of disease status in arthritis and certain cancers.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
19.9 - 300,000 pg/mL  
Limit of Detection
19.9 pg/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
MMP9
Target Class
Biomarkers
Target Species
Mouse
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
500 Assay Points

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