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AlphaLISA Mouse CCL5/RANTES Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) Detection Kit is designed for detection and quantitation of mouse CCL5/RANTES in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

This is a replacement for AL516.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL589C
Unit Size:
Part Number: AL589F
Unit Size:
  • Formats:

    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Mouse C-C Motif Chemokine 5 (CCL5), also known as Regulated upon Activation, Normal T cell Expressed and Secreted (RANTES), is an 8 kDa protein that plays a primary role in the inflammatory immune response. Human CCL5 binds to C-C chemokine receptor (CCR) types 1, 3, and 5. CCL5 dimerization and oligomerization, as well as binding to glycosaminoglycan, are key elements of cell recruitment in the inflammation process. CCL5 is a potent chemoattractant for a number of different cell types, particularly eosinophils, basophils, and mononuclear cells. CCL5 is a potential biomarker for several diseases, for example, it can be useful for indicating the magnitude of asthmatic airway inflammation. In addition to its roles as an important biomarker, CCL5 is studied for its interaction with its receptor, CCR5, in HIV research.

    AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Cytokine
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Therapeutic Area
    Inflammation

Resources

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Guide
ELISA to AlphaLISA Immunoassay Conversion Guide
elisa conversion, alphalisa, long guide