AlphaLISA Human MMP1 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Matrix Metalloproteinase-1 (MMP1) is a 52 kDa, zinc- and calcium-dependent, endopeptidase expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 is secreted as a pro-enzyme (pro-MMP1) and is activated by proteolytic cleavage by serine proteases such as plasmin. MMP1 is involved in the breakdown of all the components of the extracellular matrix during embryonic development, reproduction, and tissue remodeling. In normal conditions, MMP1 participates in tissue remodeling, blood vessel formation, and bone development. The expression level and activity of MMP1 is frequently deregulated in several diseases, cancer and rheumatoid arthritis in particular.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.