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AlphaLISA Human Leptin Detection Kit, 500 Assay Points

The AlphaLISA™ Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL225C
Unit Size: 500 Assay Points
List price: USD 2,392.00
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USD 2,392.00
USD 2,392.00 /each
Part number: AL225F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price: Login to view
USD 0.00
USD 15,950.00 /each

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Leptin is a 16-kDa hormone secreted by differentiated adipocytes. It regulates energy homeostasis as a result of its action on the brain via hypothalamic neuronal pathways expressing the leptin receptor (LR). Hereditary deficiency of leptin, or functional LRs, causes severe obesity in humans and mice. Leptin-mediated signaling has been implicated in the regulation of food intake, energy expenditure, lipid and carbohydrate metabolism, and reproductive, neuroendocrine, thyroid, and immune function.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
500
Assay Target
Leptin
Assay Target Class
Hormone
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Format
Microplates
One Unit Contains
500.0 assay points
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Metabolic
Unit Size
500 Assay Points

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