The AlphaLISA™ immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 15.6 - 1,000,000 pg/mL |
Limit of Detection | 15.6 pg/mL |
Limit of Quantification | 41.1 pg/mL |
Sample Volume | 10 µL |
The AlphaLISA™ immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
AlphaLISA features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
IRAK4 (Human Interleukin 1 Receptor Associated Kinase 4) is a serine/threonine protein kinase involved in signaling innate immune response. IRAK4 is recruited by MD88 through TLR signaling, where it phosphorylates IRAK1 and ultimately results in NF-kappa-B nuclear translocation and activation. IRAK4 supports signaling from T-cell receptors. IRAK4 contains domain structures which are similar to those of IRAK1, IRAK2, IRAKM and Pelle. IRAK4 is unique compared to IRAK1, IRAK2 and IRAKM in that it functions upstream of the other IRAKs but is more similar to Pelle in this trait. Animals deficient in IRAK-4 are more susceptible to viruses and bacteria but completely resistant to LPS challenge.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
15.6 - 1,000,000 pg/mL
|
Limit of Detection |
15.6 pg/mL
|
Limit of Quantification |
41.1 pg/mL
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
IRAK4
|
Target Class |
Biomarkers
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
|
Unit Size |
100 Assay Points
|
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