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AlphaLISA Human IRAK4 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 15.6 - 1,000,000 pg/mL  
Limit of Detection 15.6 pg/mL  
Limit of Quantification 41.1 pg/mL  
Sample Volume 10 µL

The AlphaLISA™ immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL3117HV
List Price
USD 1,131.00
Unit Size: 500 Assay Points
Part #:
AL3117C
List Price
USD 3,343.00
Unit Size: 5,000 Assay Points
Part #:
AL3117F
List Price
USD 16,680.00

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

IRAK4 (Human Interleukin 1 Receptor Associated Kinase 4) is a serine/threonine protein kinase involved in signaling innate immune response. IRAK4 is recruited by MD88 through TLR signaling, where it phosphorylates IRAK1 and ultimately results in NF-kappa-B nuclear translocation and activation. IRAK4 supports signaling from T-cell receptors. IRAK4 contains domain structures which are similar to those of IRAK1, IRAK2, IRAKM and Pelle. IRAK4 is unique compared to IRAK1, IRAK2 and IRAKM in that it functions upstream of the other IRAKs but is more similar to Pelle in this trait. Animals deficient in IRAK-4 are more susceptible to viruses and bacteria but completely resistant to LPS challenge.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
15.6 - 1,000,000 pg/mL  
Limit of Detection
15.6 pg/mL  
Limit of Quantification
41.1 pg/mL  
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
IRAK4
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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