The AlphaLISA™ Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features:
Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130/ IL-6R receptor complex, leading to activation of JAK/STAT, MAPK and PI3K cascades.
AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
5000
|
Assay Target |
IL6
|
Assay Target Class |
Cytokine
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Format |
Microplates
|
One Unit Contains |
5000.0 assay points
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Inflammation
|
Unit Size |
5,000 Assay Points
|
Dive deeper into fibrosis research
In fibrotic disorders, the normal regulation of the extracellular matrix (ECM) is compromised...
Fibrotic disorders are complex diseases believed to stem from uncontrolled and excessive wound healing processes. The exact causes...
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.