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AlphaLISA Human IL-6 Detection Kit, 5,000 Assay Points

The AlphaLISA™ Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL223C
Unit Size: 500 Assay Points
List price: USD 2,392.00
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USD 2,392.00 /each
Part number: AL223F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
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USD 15,950.00
USD 15,950.00 /each
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Part number: 6002290
List price: USD 335.60
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Part number: 6002299
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Part number: 6002350
List price: USD 355.34
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Part number: 6002359
List price: USD 1,395.00
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AlphaPlate-384,
Part number: 6005350
List price: USD 342.87
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AlphaPlate-384,
Part number: 6005359
List price: USD 1,162.00
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OptiPlate
Part number: 6007290
List price: USD 385.47
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OptiPlate
Part number: 6007299
List price: USD 1,461.00
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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half time of an ELISA assay

Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130/ IL-6R receptor complex, leading to activation of JAK/STAT, MAPK and PI3K cascades.

AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
5000
Assay Target
IL6
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Format
Microplates
One Unit Contains
5000.0 assay points
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
5,000 Assay Points

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