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AlphaLISA Human IL-18 Detection Kit, 500 Assay Points

The AlphaLISA® human Interleukin-18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

This kit is a replaced for AL241.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3137C
Unit Size: 500 Assay Points
List price: USD 2,392.00
Your price:
USD 2,392.00
USD 2,392.00 /each
Part number: AL3137F
Unit Size: 5,000 Assay Points
List price: USD 14,800.00
Your price:
USD 0.00
USD 14,800.00 /each

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin-18 (IL-18) is an 18 kDa cytokine produced by Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, and adrenal cortex cells. IL-18 is involved in the production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 works together with IL-12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharides (LPS). IL-18 is also able to induce severe inflammatory reactions, which suggests a role in certain inflammatory disorders. Elevated plasma levels of IL-18 have been reported in hematological malignancies, inflammatory diseases, and autoimmune disorders.

AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Points
500
Assay Target Class
Cytokine
Assay Technology
Alpha
Brand
AlphaLISA
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

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