AlphaLISA Human IL-11 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Human interleukin 11 (IL11) is a non-glycosylated protein of 23 kDa (179 amino acids). It is produced by bone marrow stromal cells (fibroblasts) and also by a variety of mesenchymal cells. IL11 stimulates megakaryocytopoiesis, activates osteoclasts, inhibits epithelial cell proliferation and apoptosis, and inhibits macrophage mediator production. IL-11 also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. IL11 activity is mediated by the IL-11 receptor (IL-11R) that has been reported in a wide variety of cells and tissues. IL11 binds to the IL-11R protein alone with low affinity; the affinity increases when IL-11R is associated with is signal transducer: gp130.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.