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AlphaLISA Human IL-25 Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

This product replaces AL3048.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3135HV
Unit Size: 100 Assay Points
List price: USD 945.15
Your price:
USD 945.15
USD 945.15 /each
Part number: AL3135C
Unit Size: 500 Assay Points
List price: USD 2,833.00
Your price:
USD 0.00
USD 2,833.00 /each
Part number: AL3135F
Unit Size: 5,000 assay points
List price: USD 22,660.00
Your price:
USD 0.00
USD 22,660.00 /each

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 25 (IL-25), also known as interleukin 17E is a member of the IL-17 family that is secreted by lymphocytes to stimulate the production of IL-4, IL-5 and IL-13, all mediators of inflammation. IL-25 is mainly expressed by cells in the digestive system and plays a major role in immunity of the gastro-intestinal track. However, it is also involved in inflammation of those tissues. IL-25 is also known as an anti-tumor agent in these tissues, preventing colon, pancreatic and liver cancer. This kit allows for the detection of IL-25 in biological samples.

AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Cytokine
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Autoimmunity
Inflammation
Unit Size
100 Assay Points

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