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AlphaLISA Human GDNF Detection Kit, 100 Assay Points

The AlphaLISA® Human GDNF Detection Kit is designed for detection and quantitation of human GDNF in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL319HV
Unit Size: 100 Assay Points
List price: USD 1,017.00
Your price:
USD 1,017.00
USD 1,017.00 /each
Part number: AL319C
Unit Size: 500 assay points
List price: USD 2,392.00
Your price:
USD 0.00
USD 2,392.00 /each
Part number: AL319F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price:
USD 0.00
USD 15,950.00 /each

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Glial cell-derived neurotrophic factor (GDNF) is a potent neurotrophic factor that promotes the survival of many types of neurons. GDNF is a disulphide-linked homodimer of two 134-amino acid subunits processed from a 211-amino acid precursor protein. GDNF has been observed to play a role in Parkinson's disease and amyotrophic lateral sclerosis (ALS). GDNF has also been shown to regulate kidney development and spermatogenesis and affects alcohol consumption.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Quantity in a Package Amount
1.0 Units
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Central Nervous System
Unit Size
100 Assay Points

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