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AlphaLISA Biotin-Free Human TGF-β1 Detection Kit, 100 Assay Points

The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL361HV
Unit Size: 100 Assay Points
Part Number: AL361F
Unit Size: 5,000 Assay Points
Part Number: AL361C
Unit Size: 500 assay points
  • The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

    Features:

    • No-wash steps, no separation steps
    • Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Results in less than 3 hours

    Formats:

    • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Transforming growth factor beta 1 or TGF-β1, part of the TGF β cytokine superfamily, is a 25 kD disulfide-linked homodimer. TGF-β1 is produced by many cell types including immune cells and controls cell growth, proliferation, differentiation, and apoptosis by modulating many other cytokines and cytokine receptors. Serum and urine concentrations of TGF β1 are a useful marker for determining the status of patients with diabetic nephropathy in type II.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target
    TGF-β1
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Oncology
    Unit Size
    100 Assay Points

Resources

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Application Note Icon Image
Application Note
Avoiding biotin interference in AlphaLISA assays
AlphaLISA, assays, biotin interferance