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AlphaLISA High Performance Mouse IL-1β Detection Kit, 500 Assay Points

The AlphaLISA® High Performance (HP) Mouse IL-1β Detection Kit is designed for the quantitative determination of mouse IL-1β in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL503.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL592HV
Unit Size: 100 Assay Points
List price: USD 896.90
Your price:
USD 0.00
USD 896.90 /each
Part number: AL592C
Unit Size: 500 Assay Points
List price: USD 2,261.00
Your price:
USD 2,261.00
USD 2,261.00 /each
Part number: AL592F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
Your price:
USD 0.00
USD 14,900.00 /each


Interleukin 1 beta (IL-1β) is a central player of the immune response, involved in inflammation at local and systemic levels. IL-1β is synthesized as a 31 kDa precursor that is cleaved by caspase-1 (ICE) into the active 17 kDa form and eventually released into extracellular space. Its production has been reported in many cell types including brain and monocytic and peripheral blood mononuclear cells. After binding to its receptor, IL-1RI, IL-1β triggers a cascade of kinase signaling pathways that lead to the activation of transcription factors like NFκB and AP-1, eventually activating the expression of genes such as MIP-2 and C-reactive protein.

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


Protein Analysis & Detection
Protein Detection
Assay Points
Assay Target Class
Assay Technology
Automation Compatible
Detection Method
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Therapeutic Area
Unit Size
500 Assay Points

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Cytokine AlphaLISA High performance kit flyer

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Emerging pathways to neuroinflammation and neurodegeneration

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Application Note
Homogeneous detection of cytokines from PBMC supernatants using AlphaLISA High Performance assays

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as...

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